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The in vitro assay of human bone marrow progenitors is a powerful means of studying the development of both myeloid and lymphoid lineages. The lymphoid lineage can readily be identified by the acquisition of the CD19 marker. One well-characterized murine progenitor, the common lymphoid progenitor, is able to reconstitute this CD19+ subset and competes with the myeloid lineage for the same assays, while many other murine progenitors are not. Using a combination of strategies based on flow cytometry, immunocytochemistry, and long-term immunoselection, we demonstrate the existence of a CD19+/CD34+/HLA-DR+ human progenitor that resembles the common lymphoid progenitor in mouse. The human common lymphoid progenitor is distinct from both the myeloid lineage and from the erythroid lineage, which are both CD19-/CD34-/HLA-DR- in our assays. The common lymphoid progenitor demonstrates a similar pattern of adherence to the human bone marrow stroma, whereas the erythroid lineage co-localizes with the murine macrophage progenitor on bone marrow stroma. This study demonstrates an in vitro assay system for human lymphoid progenitors and points to the fact that the human lymphoid lineage undergoes a rearrangement that bypasses the myeloid lineage.Vigorously good morning from Santa Monica
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